(A) Precipitation of Total Nucleic Acids (for all biological samples) Pellet the DNA by centrifugation at 4☌ for 10 minutes in a microcentrifuge.Resuspend the total nucleic acids in 35 µl of TE Buffer.Remove all of the residual ethanol with a pipet. Centrifuge briefly if the pellet is dislodged. Rinse twice with 70% ethanol, being careful to not dislodge the pellet.Carefully pour off the isopropanol without dislodging the pellet.Pellet the total nucleic acids by centrifugation at 4☌ for 10 minutes in a microcentrifuge.Add 500 µl of isopropanol to the recovered supernatant.Transfer the supernatant to a clean microcentrifuge tube and discard the pellet.If the resultant pellet is clear, small, or loose, add an additional 25 µl of MPC Protein Precipitation Reagent, mix, and pellet the debris again. Pellet the debris by centrifugation at 4☌ for 10 minutes at =10,000 x g in a microcentrifuge.Add 150 µl of MPC Protein Precipitation Reagent to 300 µl of lysed sample and vortex vigorously for 10 seconds.Follow MasterPure™ Protocol for lysis of fluid, cell, tissue, whole blood sample or FFPE tissue.Total Nucleic Acids, DNA or RNA Precipitation from Buffy Coat of Blood.Total Nucleic Acid Precipitation from Whole Blood Lysis.Total Nucleic Acid Precipitation from Plasma.
Total Nucleic Acid Precipitation Protocol.Precipitation Protocols using MPC Protein Precipation Solution: MPC Protein Precipitation Solutions are designed for use with the MasterPure™ kit protocols only.
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